|
KMID : 0363619980190020313
|
|
Journal of Korean Oriental Medicine
1998 Volume.19 No. 2 p.313 ~ p.325
|
|
|
The effect of Holotrichia on Natural killer Cell Activity in Mice
|
|
Kim Gi-Yeol
Kim Jong-Dae
Jung Ji-Chun
Nam Kyung-Soo
|
|
|
Abstract
|
|
|
|
The effect of Holotrichia on natural killer cell activity in normal mouse were studied. 1. The oral administration of Holotrichia increased spleen weight about 21.1% and also cell numbers of spleen compared to control mice group. 2. The cytotoxicity of effector cell was most effectively induced in a ratio of 50 : 1(effector/target cell). 3. Cytotoxicity of effector cells was. increased about 24% as compared with control group in in vivo test. 4. On the other hand, the administration of Holotrichia original solution showed significant increase the cytotoxicity. The cytotoxicity was increased concentration dependently. 5. The cytotoxicity by $^{3}H-thymidine$ incorporation assay showed similar effect with LDH enzyme method. 6. In the purified NK cells, the cytotoxicity was increased about 31% as compared with control group in in vivo system and the ratio of cytotoxicity was generally more increased than that of partially purified NK cell. 7. In vitro experimet of the purified NK cells, the cytotoxicity was increased 11.8% as compared with control group and the ratio of cytotoxicity was also more increased than that of partially purified NK cell. These results suggest that Holotrichia is administrated to mice with malignant tumors, the increase of NK cell activity may occur and affect tumor cells.
|
|
|
KEYWORD
|
|
|
Holotrichia, NK cell, LDH enzyme method, ^{3}H-thymidine incorporation assy, tumor cell, cytotoxicity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|